nuclei suspension buffer nsb Search Results


nuclei isolation buffer  (Thermo Fisher)
99
Thermo Fisher nuclei isolation buffer
Nuclei Isolation Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nuclei+suspension+buffer+nsb/Sucrose/pmc05303624-511-12-35
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loading buffer  (Thermo Fisher)
99
Thermo Fisher loading buffer
Loading Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nuclei+suspension+buffer+nsb/LDS-sample+buffer+(4X)%2C+reducing/10__1042_slash_cs20190388-55-21-23
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nuclei suspension buffer nsb  (Thermo Fisher)
99
Thermo Fisher nuclei suspension buffer nsb
Nuclei Suspension Buffer Nsb, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nuclei+suspension+buffer+nsb/TRIS-HCL/pmc10545416-261-35-63
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nuclei resuspension buffer  (Thermo Fisher)
99
Thermo Fisher nuclei resuspension buffer
Nuclei Resuspension Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nuclei+suspension+buffer+nsb/Phosphate/pmc08617297-241-31-41
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sytox green nucleic acid stain  (Thermo Fisher)
98
Thermo Fisher sytox green nucleic acid stain
FIGURE 6. Mode of cell death evoked by R-17F. A, morphological changes of Tic cells evoked by R-17F. Tic cells in single cell suspension were incubated with R-17F or R-10G (2 g/200 l of <t>1%</t> <t>BSA-PBS)</t> for 45 min at 4 °C, and morphological changes of Tic cells were monitored under a phase-contrast microscope. The arrow in the close-up view indicates a swelling phenomenon. B, comparison of cell death evoked by R-17F and by UV irradiation-induced apoptosis. a, Tic cells, which had been incubated with R-17F (10 g/100 l of medium) at 4 °C for 45 min, were incubated with <t>SYTOX</t> Green (viability staining dye) and then subjected to TO-PRO-3 staining. b, Tic cells, which had been irradiated with a UV cross-linker at 2.0 J/cm2, were cultured for 24 h, stained with SYTOX Green, and then subjected to TO-PRO-3 staining. Arrows, slight cell shrinkage and nuclear condensation. c, control cells (with no treatment). C, DNA fragmentation analysis by agarose gel electrophoresis. Tic cells in single cell suspension were either incubated with R-17F (10 g/100 l of 1% BSA-PBS) at 4 °C (lanes 1–3) or 37 °C (lanes 4–6) for 1 h (lanes 1 and 4), 3 h (lanes 2 and 5), or 5 h (lanes 3 and 6) or submitted to UV irradiation (lane 8). Lane 7, control. The molecular mass markers are shown on the left of lanes 1 and 7.
Sytox Green Nucleic Acid Stain, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nuclei+suspension+buffer+nsb/PBS/10__1074_slash_jbc__m115__657692-149-58-68
Average 98 stars, based on 1 article reviews
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fc blocking  (Becton Dickinson)
90
Becton Dickinson fc blocking
FIGURE 6. Mode of cell death evoked by R-17F. A, morphological changes of Tic cells evoked by R-17F. Tic cells in single cell suspension were incubated with R-17F or R-10G (2 g/200 l of <t>1%</t> <t>BSA-PBS)</t> for 45 min at 4 °C, and morphological changes of Tic cells were monitored under a phase-contrast microscope. The arrow in the close-up view indicates a swelling phenomenon. B, comparison of cell death evoked by R-17F and by UV irradiation-induced apoptosis. a, Tic cells, which had been incubated with R-17F (10 g/100 l of medium) at 4 °C for 45 min, were incubated with <t>SYTOX</t> Green (viability staining dye) and then subjected to TO-PRO-3 staining. b, Tic cells, which had been irradiated with a UV cross-linker at 2.0 J/cm2, were cultured for 24 h, stained with SYTOX Green, and then subjected to TO-PRO-3 staining. Arrows, slight cell shrinkage and nuclear condensation. c, control cells (with no treatment). C, DNA fragmentation analysis by agarose gel electrophoresis. Tic cells in single cell suspension were either incubated with R-17F (10 g/100 l of 1% BSA-PBS) at 4 °C (lanes 1–3) or 37 °C (lanes 4–6) for 1 h (lanes 1 and 4), 3 h (lanes 2 and 5), or 5 h (lanes 3 and 6) or submitted to UV irradiation (lane 8). Lane 7, control. The molecular mass markers are shown on the left of lanes 1 and 7.
Fc Blocking, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nuclei+suspension+buffer+nsb/anti+cd56/pmc10864800-379-11-14
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sds polyacrylamide gel electrophoresis  (Bio-Rad)
98
Bio-Rad sds polyacrylamide gel electrophoresis
FIGURE 6. Mode of cell death evoked by R-17F. A, morphological changes of Tic cells evoked by R-17F. Tic cells in single cell suspension were incubated with R-17F or R-10G (2 g/200 l of <t>1%</t> <t>BSA-PBS)</t> for 45 min at 4 °C, and morphological changes of Tic cells were monitored under a phase-contrast microscope. The arrow in the close-up view indicates a swelling phenomenon. B, comparison of cell death evoked by R-17F and by UV irradiation-induced apoptosis. a, Tic cells, which had been incubated with R-17F (10 g/100 l of medium) at 4 °C for 45 min, were incubated with <t>SYTOX</t> Green (viability staining dye) and then subjected to TO-PRO-3 staining. b, Tic cells, which had been irradiated with a UV cross-linker at 2.0 J/cm2, were cultured for 24 h, stained with SYTOX Green, and then subjected to TO-PRO-3 staining. Arrows, slight cell shrinkage and nuclear condensation. c, control cells (with no treatment). C, DNA fragmentation analysis by agarose gel electrophoresis. Tic cells in single cell suspension were either incubated with R-17F (10 g/100 l of 1% BSA-PBS) at 4 °C (lanes 1–3) or 37 °C (lanes 4–6) for 1 h (lanes 1 and 4), 3 h (lanes 2 and 5), or 5 h (lanes 3 and 6) or submitted to UV irradiation (lane 8). Lane 7, control. The molecular mass markers are shown on the left of lanes 1 and 7.
Sds Polyacrylamide Gel Electrophoresis, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 98 stars, based on 1 article reviews
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nuclei lysis solution  (Promega)
90
Promega nuclei lysis solution
FIGURE 6. Mode of cell death evoked by R-17F. A, morphological changes of Tic cells evoked by R-17F. Tic cells in single cell suspension were incubated with R-17F or R-10G (2 g/200 l of <t>1%</t> <t>BSA-PBS)</t> for 45 min at 4 °C, and morphological changes of Tic cells were monitored under a phase-contrast microscope. The arrow in the close-up view indicates a swelling phenomenon. B, comparison of cell death evoked by R-17F and by UV irradiation-induced apoptosis. a, Tic cells, which had been incubated with R-17F (10 g/100 l of medium) at 4 °C for 45 min, were incubated with <t>SYTOX</t> Green (viability staining dye) and then subjected to TO-PRO-3 staining. b, Tic cells, which had been irradiated with a UV cross-linker at 2.0 J/cm2, were cultured for 24 h, stained with SYTOX Green, and then subjected to TO-PRO-3 staining. Arrows, slight cell shrinkage and nuclear condensation. c, control cells (with no treatment). C, DNA fragmentation analysis by agarose gel electrophoresis. Tic cells in single cell suspension were either incubated with R-17F (10 g/100 l of 1% BSA-PBS) at 4 °C (lanes 1–3) or 37 °C (lanes 4–6) for 1 h (lanes 1 and 4), 3 h (lanes 2 and 5), or 5 h (lanes 3 and 6) or submitted to UV irradiation (lane 8). Lane 7, control. The molecular mass markers are shown on the left of lanes 1 and 7.
Nuclei Lysis Solution, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nuclei+suspension+buffer+nsb/nuclei+lysis+solution/pm35708333-201-14-19
Average 90 stars, based on 1 article reviews
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dna hydration solution  (Qiagen)
92
Qiagen dna hydration solution
FIGURE 6. Mode of cell death evoked by R-17F. A, morphological changes of Tic cells evoked by R-17F. Tic cells in single cell suspension were incubated with R-17F or R-10G (2 g/200 l of <t>1%</t> <t>BSA-PBS)</t> for 45 min at 4 °C, and morphological changes of Tic cells were monitored under a phase-contrast microscope. The arrow in the close-up view indicates a swelling phenomenon. B, comparison of cell death evoked by R-17F and by UV irradiation-induced apoptosis. a, Tic cells, which had been incubated with R-17F (10 g/100 l of medium) at 4 °C for 45 min, were incubated with <t>SYTOX</t> Green (viability staining dye) and then subjected to TO-PRO-3 staining. b, Tic cells, which had been irradiated with a UV cross-linker at 2.0 J/cm2, were cultured for 24 h, stained with SYTOX Green, and then subjected to TO-PRO-3 staining. Arrows, slight cell shrinkage and nuclear condensation. c, control cells (with no treatment). C, DNA fragmentation analysis by agarose gel electrophoresis. Tic cells in single cell suspension were either incubated with R-17F (10 g/100 l of 1% BSA-PBS) at 4 °C (lanes 1–3) or 37 °C (lanes 4–6) for 1 h (lanes 1 and 4), 3 h (lanes 2 and 5), or 5 h (lanes 3 and 6) or submitted to UV irradiation (lane 8). Lane 7, control. The molecular mass markers are shown on the left of lanes 1 and 7.
Dna Hydration Solution, supplied by Qiagen, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nuclei+suspension+buffer+nsb/DNA+Hydration+Solution/us11680284-115-12-11
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nuclei buffer  (10X Genomics)
86
10X Genomics nuclei buffer
FIGURE 6. Mode of cell death evoked by R-17F. A, morphological changes of Tic cells evoked by R-17F. Tic cells in single cell suspension were incubated with R-17F or R-10G (2 g/200 l of <t>1%</t> <t>BSA-PBS)</t> for 45 min at 4 °C, and morphological changes of Tic cells were monitored under a phase-contrast microscope. The arrow in the close-up view indicates a swelling phenomenon. B, comparison of cell death evoked by R-17F and by UV irradiation-induced apoptosis. a, Tic cells, which had been incubated with R-17F (10 g/100 l of medium) at 4 °C for 45 min, were incubated with <t>SYTOX</t> Green (viability staining dye) and then subjected to TO-PRO-3 staining. b, Tic cells, which had been irradiated with a UV cross-linker at 2.0 J/cm2, were cultured for 24 h, stained with SYTOX Green, and then subjected to TO-PRO-3 staining. Arrows, slight cell shrinkage and nuclear condensation. c, control cells (with no treatment). C, DNA fragmentation analysis by agarose gel electrophoresis. Tic cells in single cell suspension were either incubated with R-17F (10 g/100 l of 1% BSA-PBS) at 4 °C (lanes 1–3) or 37 °C (lanes 4–6) for 1 h (lanes 1 and 4), 3 h (lanes 2 and 5), or 5 h (lanes 3 and 6) or submitted to UV irradiation (lane 8). Lane 7, control. The molecular mass markers are shown on the left of lanes 1 and 7.
Nuclei Buffer, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nuclei+suspension+buffer+nsb/buffer+nuclei/bio_rxiv__64898__2026__03__31__715694-141-9-16
Average 86 stars, based on 1 article reviews
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chef mammalian dna extraction kit  (Bio-Rad)
97
Bio-Rad chef mammalian dna extraction kit
FIGURE 6. Mode of cell death evoked by R-17F. A, morphological changes of Tic cells evoked by R-17F. Tic cells in single cell suspension were incubated with R-17F or R-10G (2 g/200 l of <t>1%</t> <t>BSA-PBS)</t> for 45 min at 4 °C, and morphological changes of Tic cells were monitored under a phase-contrast microscope. The arrow in the close-up view indicates a swelling phenomenon. B, comparison of cell death evoked by R-17F and by UV irradiation-induced apoptosis. a, Tic cells, which had been incubated with R-17F (10 g/100 l of medium) at 4 °C for 45 min, were incubated with <t>SYTOX</t> Green (viability staining dye) and then subjected to TO-PRO-3 staining. b, Tic cells, which had been irradiated with a UV cross-linker at 2.0 J/cm2, were cultured for 24 h, stained with SYTOX Green, and then subjected to TO-PRO-3 staining. Arrows, slight cell shrinkage and nuclear condensation. c, control cells (with no treatment). C, DNA fragmentation analysis by agarose gel electrophoresis. Tic cells in single cell suspension were either incubated with R-17F (10 g/100 l of 1% BSA-PBS) at 4 °C (lanes 1–3) or 37 °C (lanes 4–6) for 1 h (lanes 1 and 4), 3 h (lanes 2 and 5), or 5 h (lanes 3 and 6) or submitted to UV irradiation (lane 8). Lane 7, control. The molecular mass markers are shown on the left of lanes 1 and 7.
Chef Mammalian Dna Extraction Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chef mammalian dna extraction kit - by Bioz Stars, 2026-10
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nuclei lysis buffer  (DIAGENODE DIAGNOSTICS)
90
DIAGENODE DIAGNOSTICS nuclei lysis buffer
FIGURE 6. Mode of cell death evoked by R-17F. A, morphological changes of Tic cells evoked by R-17F. Tic cells in single cell suspension were incubated with R-17F or R-10G (2 g/200 l of <t>1%</t> <t>BSA-PBS)</t> for 45 min at 4 °C, and morphological changes of Tic cells were monitored under a phase-contrast microscope. The arrow in the close-up view indicates a swelling phenomenon. B, comparison of cell death evoked by R-17F and by UV irradiation-induced apoptosis. a, Tic cells, which had been incubated with R-17F (10 g/100 l of medium) at 4 °C for 45 min, were incubated with <t>SYTOX</t> Green (viability staining dye) and then subjected to TO-PRO-3 staining. b, Tic cells, which had been irradiated with a UV cross-linker at 2.0 J/cm2, were cultured for 24 h, stained with SYTOX Green, and then subjected to TO-PRO-3 staining. Arrows, slight cell shrinkage and nuclear condensation. c, control cells (with no treatment). C, DNA fragmentation analysis by agarose gel electrophoresis. Tic cells in single cell suspension were either incubated with R-17F (10 g/100 l of 1% BSA-PBS) at 4 °C (lanes 1–3) or 37 °C (lanes 4–6) for 1 h (lanes 1 and 4), 3 h (lanes 2 and 5), or 5 h (lanes 3 and 6) or submitted to UV irradiation (lane 8). Lane 7, control. The molecular mass markers are shown on the left of lanes 1 and 7.
Nuclei Lysis Buffer, supplied by DIAGENODE DIAGNOSTICS, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


FIGURE 6. Mode of cell death evoked by R-17F. A, morphological changes of Tic cells evoked by R-17F. Tic cells in single cell suspension were incubated with R-17F or R-10G (2 g/200 l of 1% BSA-PBS) for 45 min at 4 °C, and morphological changes of Tic cells were monitored under a phase-contrast microscope. The arrow in the close-up view indicates a swelling phenomenon. B, comparison of cell death evoked by R-17F and by UV irradiation-induced apoptosis. a, Tic cells, which had been incubated with R-17F (10 g/100 l of medium) at 4 °C for 45 min, were incubated with SYTOX Green (viability staining dye) and then subjected to TO-PRO-3 staining. b, Tic cells, which had been irradiated with a UV cross-linker at 2.0 J/cm2, were cultured for 24 h, stained with SYTOX Green, and then subjected to TO-PRO-3 staining. Arrows, slight cell shrinkage and nuclear condensation. c, control cells (with no treatment). C, DNA fragmentation analysis by agarose gel electrophoresis. Tic cells in single cell suspension were either incubated with R-17F (10 g/100 l of 1% BSA-PBS) at 4 °C (lanes 1–3) or 37 °C (lanes 4–6) for 1 h (lanes 1 and 4), 3 h (lanes 2 and 5), or 5 h (lanes 3 and 6) or submitted to UV irradiation (lane 8). Lane 7, control. The molecular mass markers are shown on the left of lanes 1 and 7.

Journal: Journal of Biological Chemistry

Article Title: A Cytotoxic Antibody Recognizing Lacto-N-fucopentaose I (LNFP I) on Human Induced Pluripotent Stem (hiPS) Cells

doi: 10.1074/jbc.m115.657692

Figure Lengend Snippet: FIGURE 6. Mode of cell death evoked by R-17F. A, morphological changes of Tic cells evoked by R-17F. Tic cells in single cell suspension were incubated with R-17F or R-10G (2 g/200 l of 1% BSA-PBS) for 45 min at 4 °C, and morphological changes of Tic cells were monitored under a phase-contrast microscope. The arrow in the close-up view indicates a swelling phenomenon. B, comparison of cell death evoked by R-17F and by UV irradiation-induced apoptosis. a, Tic cells, which had been incubated with R-17F (10 g/100 l of medium) at 4 °C for 45 min, were incubated with SYTOX Green (viability staining dye) and then subjected to TO-PRO-3 staining. b, Tic cells, which had been irradiated with a UV cross-linker at 2.0 J/cm2, were cultured for 24 h, stained with SYTOX Green, and then subjected to TO-PRO-3 staining. Arrows, slight cell shrinkage and nuclear condensation. c, control cells (with no treatment). C, DNA fragmentation analysis by agarose gel electrophoresis. Tic cells in single cell suspension were either incubated with R-17F (10 g/100 l of 1% BSA-PBS) at 4 °C (lanes 1–3) or 37 °C (lanes 4–6) for 1 h (lanes 1 and 4), 3 h (lanes 2 and 5), or 5 h (lanes 3 and 6) or submitted to UV irradiation (lane 8). Lane 7, control. The molecular mass markers are shown on the left of lanes 1 and 7.

Article Snippet: Confocal Scanning Microscopy—For UV irradiation-induced apoptosis, Tic cells cultured in Petri dishes containing the hiPS culture medium (1 ml) were irradiated with SpectroLinker XL-1000 UV cross-linker (Spectronics Corp. Westbury, NY) at 2.0 J/cm2 and then cultured in the hiPS culture medium at 37 °C/5% CO2 for 24 h. After washing in PBS, the collected cells were incubated with SYTOX Green nucleic acid stain (1 M in 1% BSA-PBS; Life Technologies, Inc.) at room temperature for 30 min. After washing in 1% BSA-PBS, the cells were transferred to chamber slides and fixed with 0.5 ml of 4% PFA for 30 min. Then the cells were incubated with TOPRO-3 for 1 h and visualized under a laser confocal scanning microscope.

Techniques: Suspension, Incubation, Microscopy, Comparison, Irradiation, Staining, Cell Culture, Control, Agarose Gel Electrophoresis